Analysis of siRNA with Denaturing and Non-Denaturing Ion-Pair Reversed-Phase Liquid Chromatography Methods

In this paper, we describe denaturing and non-denaturing ion-pair reversed-phase liquid chromatography (RPLC) methods for analyzing small interfering ribonucleic acid (siRNA). Drug formulations consisting of one or two siRNA duplexes were analyzed in conditions at a low column temperature to separate intact from single-stranded oligonucleotide contaminants quantify them. method, used an elevated ensure the denaturation into their counterparts. The goal LC method was investigate impurities daughter oligonucleotides siRNA. A with chemically modified C18 hardware showed improvements analytical performance nucleic acids compared conventional stainless-steel same pore size.